Coding

Part:BBa_K1705001

Designed by: Nathan Hunt   Group: iGEM15_MIT   (2015-09-17)

MoClo atfA

atfA is a wax ester synthetase/acyl-CoA diacylglycerol acyltransferase gene from Acinetobacter sp. ADP1. In its functionality as an acyltransferase, it catalyzes the reaction between ethanol and a fatty acyl-CoA to form a fatty acid ethyl ester (FAEE), a form of biodiesel. When combined with a pyruvate decarboxylase (pdc) and an alcohol dehydrogenase (adhB), this gene can be used to insert a pathway for production of biodiesel.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 131
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1
    Illegal BsaI.rc site found at 1391


[edit]
Categories
Parameters
None